sRNA sequencing of L. case BL23 - SRA

SRX9518718: sRNA sequencing of L. case BL23
1 ILLUMINA (NextSeq 500) run: 17.4M spots, 2.6G bases, 978.8Mb downloads

Design: Total RNR was extracted using RNAzol RT (RN190) (Molecular Research Center, Inc.) from L. casei BL23 grown at 37 C in BD Difco Lactobacilli MRS Broth collected at six different growth points and treated with DNaseI, RNase free (Thermo Scientific). rRNAs were depleted using modified RiboMinus Transcriptome Isolation Kit, bacteria (Invitrogen) and 50-500 nt RNA fraction was retrieved after size selection using 8 % denaturing PAA gel. After treatment with RNA 5 Pyrophosphohydrolase (RppH) (New England Biolabs) three biological replicates of C and U sRNA libraries were prepared combining the protocols of mDOT-seq and NEXTflex Small RNA-Seq Kit v3 (PerkinElmer), while for N libraries RNA was processed only according to the later one. All libraries were pooled together and sequenced at Lexogen facilities using NextSeq 500/550 Mid Output Kit to obtain 2 75 paired-end reads.

Submitted by: Vilnius University

Study: sRNA sequencing of Lactobacillus casei BL23

PRJNA678647 • SRP292855 • All experiments • All runs

show Abstracthide Abstract

Total RNR was extracted using RNAzol RT (RN190) (Molecular Research Center,Inc.) from L. casei BL23 grown at 37 C in BD (TM) Difc (TM) Lactobacilli MRS Broth collected at six different growth points and treated with DNaseI, RNase free (Thermo Scientific). rRNAs were depleted using modified RiboMinus Transcriptome Isolation Kit, bacteria (Invitrogen) and 50-500 nt RNA fraction was retrieved after size selection using 8 % denaturing PAA gel. After treatment with RNA 5' Pyrophosphohydrolase (RppH) (New England Biolabs) three biological replicates of C and U sRNA libraries were prepared combining the protocols of mDOT-seq and NEXTflex Small RNA-Seq Kit v3 (PerkinElmer), while for N libraries RNA was processed only according to the later one. All libraries were pooled together and sequenced at Lexogen facilities using NextSeq 500/550 Mid Output Kit to obtain 2 x 75 paired-end reads.

Sample:

SAMN16815319 • SRS7726339 • All experiments • All runs

Organism: Lacticaseibacillus casei BL23

Library:

Name: N1

Instrument: NextSeq 500

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: size fractionation

Layout: PAIRED

Runs: 1 run, 17.4M spots, 2.6G bases, 978.8Mb

Run	# of Spots	# of Bases	Size	Published
SRR13071197	17,385,572	2.6G	978.8Mb	2021-05-20

ID:: 12438506

SRA

Sequence Read Archive

Result Filters

Send to:

Supplemental Content

Related information

Recent activity